Preparation and evaluation of Necrotic enteritis (NE) vaccine using alpha toxin with different adjuvants in broilers.
نویسندگان
چکیده
ABSTRACT In the present study we used one hundred and twenty-five broiler chicks (Arbo) one- day old as experimental birds, were divided into five groups each of chicks. The first group kept control, 2nd was non-vaccinated challenged, 3rd infected with Emeria tenella at 15th days age 4th vaccinated 5th challenged. in 4 by locally prepared NE vaccine adjuvinated Aluminum hydroxide dose 0.5ml subcutaneously seven boostered eighteen days. While Montanide oil. challenged (2, 3, 5) 27, 28 29 C. perfringes type A whole culture 3ml contains 109 Cfu. Serum samples collected after challenge thirty-five results showed that higher immune response, lower mortality rate, increase body weight low feed conversion rate 5 compared to (1, 2, 3 4).
منابع مشابه
Probiotic Enhanced Intestinal Immunity in Broilers against Subclinical Necrotic Enteritis
Along with banning of antibiotics, necrotic enteritis (NE), especially subclinical NE (SNE) whereby no clinical signs are present in chicks, has become one of the most threatening problems in poultry industry. Therefore, increasing attention has been focused on research and application of effective probiotic strains, as an alternative to antibiotics, to prevent SNE in broilers. In the present s...
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The Clostridium perfringens alpha-toxin has previously been implicated as the major virulence factor in necrotic enteritis in chickens, although definitive proof has not been reported. In this study an alpha-toxin mutant was constructed in a virulent chicken isolate and shown to retain full virulence in a chicken disease model. These results demonstrated that alpha-toxin is not an essential vir...
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ژورنال
عنوان ژورنال: Benha Veterinary Medical Journal
سال: 2021
ISSN: ['1110-6581']
DOI: https://doi.org/10.21608/bvmj.2021.57081.1326